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ultra lowadhesion plates  (Greiner Bio)


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    Greiner Bio ultra lowadhesion plates
    Ultra Lowadhesion Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 95/100, based on 227 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/suspension+culture+plate/Multiwell+Plate+For+Suspension+Culture+24+Well/pm41923644-932-25-27
    Average 95 stars, based on 227 article reviews
    ultra lowadhesion plates - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Prostate cancer associated fibroblasts have distinct morphomechanical features that are associated with patient outcome
    Article Snippet: Fibroblasts were washed with PBS and detached with TrypLE Express (Gibco). .. Thereafter, cells were pelleted by centrifugation, resuspended in 0.8mL CO 2 -independent medium (Gibco) with 5 % (v/v) FBS and kept in a suspension culture plate (Greiner) for 1 h at room temperature. ..

    Suspension:

    Article Title: Prostate cancer associated fibroblasts have distinct morphomechanical features that are associated with patient outcome
    Article Snippet: Fibroblasts were washed with PBS and detached with TrypLE Express (Gibco). .. Thereafter, cells were pelleted by centrifugation, resuspended in 0.8mL CO 2 -independent medium (Gibco) with 5 % (v/v) FBS and kept in a suspension culture plate (Greiner) for 1 h at room temperature. ..

    Article Title: Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation.
    Article Snippet: Biological safety cabinet (e.g., Thermo Fisher Scientific, cat. no. 1316) Safety bunsen burner for safety cabinets (optional) Cell culture incubator with 5% CO2, 37 °C (e.g., Panasonic, cat. no. MCO-18AC-PE) Bright-field microscope (e.g., Biocompare, cat. no. TS100/TS100F) High-speed centrifuge (e.g., Eppendorf, cat. no. 5810 R) Water bath 37 °C (Thermo Fisher Scientific, cat. no. BLE-300-010T) Temperature-controlled shaker set to 37 °C (e.g., LT-X (Lab-Therm), cat. no. SMX1700) Fine scissors (Fine Science Tools, cat. no. 91460-11) Two fine forceps (Fisher, cat. no. DKC-770-R) Pipette aid, serological pipettes (10 ml; VWR, cat. no. 734-1738) Glass Pasteur pipette (150-mm length, unplugged; Thermo Fisher Scientific, cat. no. 10006021) Calibrated pipettes (P10, P1,000, P200, P20 and P2—e.g., Sigma-Adrich, cat. no. Z740362) and sterilized pipette tips. .. When you are working with viruses, use filter tips (Starlab, 0.1- to 10-μl filter tips, cat. no. S1120-3810; 20-μl filter tips, cat. no. S1120-1810; 100- to 200-μl filter tips, cat. no. S1120-8810; racked, 100- to 1,000-μl filter tips, cat. no. S1122-1830) Conical centrifuge tubes (SLS, 15 ml, cat. no. 352096, and 50 ml, cat. no. 352070) Microcentrifuge tubes (1.5 ml, Thermo Fisher Scientific, cat. no. 30125150) FACS tubes (or other tubes suitable for the cell sorter; e.g., BD falcon, cat. no. 352063) 70-μm Cell strainer (Falcon, cat. no. 352350) 40-μm Cell strainer (Falcon, cat. no. 352340) 100-mm Petri dish (VWR, cat. no. 470151-288) 48-Well suspension culture plate (GreinerBio-one, cat. no. 677 102) 24-Well suspension culture plate (GreinerBio-one, cat. no. 662 102) 24-Well adherent culture plate (Thermo Scientific Nunclon, cat. no. 142475) Cell freezing container (CoolCell LX; Biocision, cat. no. BCS-405) 2-ml Cryogenic vials (VWR, cat. no. 479-0287) Standard hemocytometer (ImmuneSystems, cat. no. BVS100 or Sigma-Aldrich, cat. no. BR719520) Dry ice Ice Equipment for processing organoids for analysis 7-ml Plastic Pasteur pipettes (VWR, cat. no. 612-1681) 5-ml Flat-bottom glass tubes (Thermo Fisher Scientific, cat. no. 11563542) Incubator prewarmed to 65 °C (Memmert Incubator, cat. no. LAB129) Tissue cassette (VWR, cat. no. 720-0228) Glass slides (VWR, cat. no. 631-9483) • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • © 20 16 N at u re A m er ic a, In c. A ll ri g h ts r es er ve d . nature protocols | VOL.11 NO.9 | 2016 | 1731 Rspo1-conditioned medium, 10 mM nicotinamide, 10 nM recombinant human [Leu15]-gastrin I, 50 ng/ml recombinant mouse EGF, 100 ng/ml recombinant human FGF10 and 25 ng/ml recombinant human Noggin or 5% (vol/vol) Noggin-conditioned medium. ..

    Article Title: Patient-derived tumor organoids with p53 mutations, and not wild-type p53, are sensitive to synergistic combination PARP inhibitor treatment.
    Article Snippet: .. The final resuspended pellet was combined with Matrigel (Corning) in a 1:2 volume media:Matrigel, with 5 80 μl droplets pipetted onto each well of a six-well Jo urn al Pr e-p roo f 8 suspension culture plate (Greiner Bio-One). ..

    Article Title: Assessing the origin of high-grade serous ovarian cancer using CRISPR-modification of mouse organoids
    Article Snippet: The tissue was incubated at 37 °C for 2 h while shaking followed by vigorous mechanical sharing using a fire-polished glass pipette and centrifugation for 5 min at 450 × g . The material was further digested with TrypLE (Gibco, Cat. 12605-010) for 5 min at 37 °C, washed several times with ice-cold AdDF+++ and embedded in Basement Membrane Extract (Cultrex ® BME RGF type 2, Amsbio, Cat. 3533-005-02). .. The cell-BME suspension was plated on a pre-warmed suspension culture plate (Greiner) and allowed to solidify at 37 °C for 30 min before addition of medium. .. The basal culture medium for oviductal organoids includes 20% R-spondin conditioned medium (made in-house), 1% Noggin conditioned medium (U Protein Express), 1x B27 (Gibco), 1.25 mM n-Acetylcystein (Sigma), 50 ng/ml EGF (Peprotech), and 500 nM A83-01 (Tocris).

    FACS:

    Article Title: Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation.
    Article Snippet: Biological safety cabinet (e.g., Thermo Fisher Scientific, cat. no. 1316) Safety bunsen burner for safety cabinets (optional) Cell culture incubator with 5% CO2, 37 °C (e.g., Panasonic, cat. no. MCO-18AC-PE) Bright-field microscope (e.g., Biocompare, cat. no. TS100/TS100F) High-speed centrifuge (e.g., Eppendorf, cat. no. 5810 R) Water bath 37 °C (Thermo Fisher Scientific, cat. no. BLE-300-010T) Temperature-controlled shaker set to 37 °C (e.g., LT-X (Lab-Therm), cat. no. SMX1700) Fine scissors (Fine Science Tools, cat. no. 91460-11) Two fine forceps (Fisher, cat. no. DKC-770-R) Pipette aid, serological pipettes (10 ml; VWR, cat. no. 734-1738) Glass Pasteur pipette (150-mm length, unplugged; Thermo Fisher Scientific, cat. no. 10006021) Calibrated pipettes (P10, P1,000, P200, P20 and P2—e.g., Sigma-Adrich, cat. no. Z740362) and sterilized pipette tips. .. When you are working with viruses, use filter tips (Starlab, 0.1- to 10-μl filter tips, cat. no. S1120-3810; 20-μl filter tips, cat. no. S1120-1810; 100- to 200-μl filter tips, cat. no. S1120-8810; racked, 100- to 1,000-μl filter tips, cat. no. S1122-1830) Conical centrifuge tubes (SLS, 15 ml, cat. no. 352096, and 50 ml, cat. no. 352070) Microcentrifuge tubes (1.5 ml, Thermo Fisher Scientific, cat. no. 30125150) FACS tubes (or other tubes suitable for the cell sorter; e.g., BD falcon, cat. no. 352063) 70-μm Cell strainer (Falcon, cat. no. 352350) 40-μm Cell strainer (Falcon, cat. no. 352340) 100-mm Petri dish (VWR, cat. no. 470151-288) 48-Well suspension culture plate (GreinerBio-one, cat. no. 677 102) 24-Well suspension culture plate (GreinerBio-one, cat. no. 662 102) 24-Well adherent culture plate (Thermo Scientific Nunclon, cat. no. 142475) Cell freezing container (CoolCell LX; Biocision, cat. no. BCS-405) 2-ml Cryogenic vials (VWR, cat. no. 479-0287) Standard hemocytometer (ImmuneSystems, cat. no. BVS100 or Sigma-Aldrich, cat. no. BR719520) Dry ice Ice Equipment for processing organoids for analysis 7-ml Plastic Pasteur pipettes (VWR, cat. no. 612-1681) 5-ml Flat-bottom glass tubes (Thermo Fisher Scientific, cat. no. 11563542) Incubator prewarmed to 65 °C (Memmert Incubator, cat. no. LAB129) Tissue cassette (VWR, cat. no. 720-0228) Glass slides (VWR, cat. no. 631-9483) • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • © 20 16 N at u re A m er ic a, In c. A ll ri g h ts r es er ve d . nature protocols | VOL.11 NO.9 | 2016 | 1731 Rspo1-conditioned medium, 10 mM nicotinamide, 10 nM recombinant human [Leu15]-gastrin I, 50 ng/ml recombinant mouse EGF, 100 ng/ml recombinant human FGF10 and 25 ng/ml recombinant human Noggin or 5% (vol/vol) Noggin-conditioned medium. ..

    Recombinant:

    Article Title: Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation.
    Article Snippet: Biological safety cabinet (e.g., Thermo Fisher Scientific, cat. no. 1316) Safety bunsen burner for safety cabinets (optional) Cell culture incubator with 5% CO2, 37 °C (e.g., Panasonic, cat. no. MCO-18AC-PE) Bright-field microscope (e.g., Biocompare, cat. no. TS100/TS100F) High-speed centrifuge (e.g., Eppendorf, cat. no. 5810 R) Water bath 37 °C (Thermo Fisher Scientific, cat. no. BLE-300-010T) Temperature-controlled shaker set to 37 °C (e.g., LT-X (Lab-Therm), cat. no. SMX1700) Fine scissors (Fine Science Tools, cat. no. 91460-11) Two fine forceps (Fisher, cat. no. DKC-770-R) Pipette aid, serological pipettes (10 ml; VWR, cat. no. 734-1738) Glass Pasteur pipette (150-mm length, unplugged; Thermo Fisher Scientific, cat. no. 10006021) Calibrated pipettes (P10, P1,000, P200, P20 and P2—e.g., Sigma-Adrich, cat. no. Z740362) and sterilized pipette tips. .. When you are working with viruses, use filter tips (Starlab, 0.1- to 10-μl filter tips, cat. no. S1120-3810; 20-μl filter tips, cat. no. S1120-1810; 100- to 200-μl filter tips, cat. no. S1120-8810; racked, 100- to 1,000-μl filter tips, cat. no. S1122-1830) Conical centrifuge tubes (SLS, 15 ml, cat. no. 352096, and 50 ml, cat. no. 352070) Microcentrifuge tubes (1.5 ml, Thermo Fisher Scientific, cat. no. 30125150) FACS tubes (or other tubes suitable for the cell sorter; e.g., BD falcon, cat. no. 352063) 70-μm Cell strainer (Falcon, cat. no. 352350) 40-μm Cell strainer (Falcon, cat. no. 352340) 100-mm Petri dish (VWR, cat. no. 470151-288) 48-Well suspension culture plate (GreinerBio-one, cat. no. 677 102) 24-Well suspension culture plate (GreinerBio-one, cat. no. 662 102) 24-Well adherent culture plate (Thermo Scientific Nunclon, cat. no. 142475) Cell freezing container (CoolCell LX; Biocision, cat. no. BCS-405) 2-ml Cryogenic vials (VWR, cat. no. 479-0287) Standard hemocytometer (ImmuneSystems, cat. no. BVS100 or Sigma-Aldrich, cat. no. BR719520) Dry ice Ice Equipment for processing organoids for analysis 7-ml Plastic Pasteur pipettes (VWR, cat. no. 612-1681) 5-ml Flat-bottom glass tubes (Thermo Fisher Scientific, cat. no. 11563542) Incubator prewarmed to 65 °C (Memmert Incubator, cat. no. LAB129) Tissue cassette (VWR, cat. no. 720-0228) Glass slides (VWR, cat. no. 631-9483) • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • © 20 16 N at u re A m er ic a, In c. A ll ri g h ts r es er ve d . nature protocols | VOL.11 NO.9 | 2016 | 1731 Rspo1-conditioned medium, 10 mM nicotinamide, 10 nM recombinant human [Leu15]-gastrin I, 50 ng/ml recombinant mouse EGF, 100 ng/ml recombinant human FGF10 and 25 ng/ml recombinant human Noggin or 5% (vol/vol) Noggin-conditioned medium. ..



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